Composite

Part:BBa_J15509:Design

Designed by: Kwabena O. Duedu, Chris French, Sahreena Lakhundi   Group: French Lab   (2013-11-25)


Plac+RBS+cex+RBS+cenA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1134
    Illegal NotI site found at 2115
    Illegal NotI site found at 3259
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3172
    Illegal BamHI site found at 2308
    Illegal XhoI site found at 2670
    Illegal XhoI site found at 2919
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1642
    Illegal NgoMIV site found at 2450
    Illegal NgoMIV site found at 3375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2354
    Illegal BsaI.rc site found at 1187
    Illegal SapI.rc site found at 1270


Design Notes

Cex is a GC rich part and may require extended denaturation in amplifying the gene.


Source

Cellulomonas fimi

References